Certain mouse strains are deficient in a kidney brush-border metallo-endopeptidase activity.
نویسندگان
چکیده
Preparations of microvilli from kidneys of BALB/c mice contain an alkaline metallo-endopeptidase, meprin (metallo-endopeptidase from renal tissue). Certain genealogically related inbred mice are markedly deficient in meprin activity. The meprin-deficient strains (CBA/J and C3H/HeJ) exhibit normal levels of other brush-border enzymes: alkaline phosphatase, aminopeptidase M and another proteinase, a phosphoramidon-sensitive neutral endopeptidase. Meprin deficiency cannot be attributed to a shift in pH optimum and is unlikely to be due to the presence of endogenous inhibitors.
منابع مشابه
Mep-1 gene controlling a kidney metalloendopeptidase is linked to the major histocompatibility complex in mice.
Meprin, a glycoprotein with potent metalloendopeptidase activity, is an integral component of the brush border membrane of mouse kidney. Previously we reported that genealogically related inbred mouse strains (C3H and CBA) are markedly deficient in the activity of this enzyme. We report here that meprin deficiency is inherited as an autosomal recessive trait and that several other inbred strain...
متن کاملSex and strain differences in the brush border and PAS-positive granules and giant bodies of the mouse renal S3 segment cells.
We recently demonstrated sexual dimorphism in the S3 segment of the ICR mouse kidney, as differences in periodic acid Schiff (PAS) staining on the brush border and the number and size of PAS-positive granules. However, whether these sex dependent features in the S3 segment of the mouse kidney occur only in the ICR strain or are a general feature also observed in other strains is unclear. In the...
متن کاملFusion of a cleavable signal peptide to the ectodomain of neutral endopeptidase (EC 3.4.24.11) results in the secretion of an active enzyme in COS-1 cells.
Neutral endopeptidase (EC 3.4.24.11) is an integral membrane protein found at the plasma membrane of many cell types and is especially abundant at the apical "brush border" membrane of the kidney proximal tubules. The enzyme consists of a short amino-terminal cytosolic domain of 27 amino acids, a single hydrophobic sequence which is believed to be responsible for anchoring the enzyme in the pla...
متن کاملBrush border enzymes in coeliac disease: histochemical evaluation.
Two hundred and ninety four duodenal and jejunal mucosal biopsy specimens from patients with coeliac disease, treated and untreated, and other conditions were examined histologically and by histochemical staining for five peptidase and three disaccharidase enzymes to determine profiles of activity. Suppression of activity paralleled the histology with the following enzymes: lactase, trehalase, ...
متن کاملUltra Structure of Excretory Organ (Kidney) in Juvenile Grouper, Epinephelus coioides
In order to study of kidney tubules, twenty specimen of 45 days old juvenile grouper (Epinephelus coioides) were prepared after proper biometry. Five mm thick sections were removed from the middle and posterior end of the kidney and fixed in Bouin and glutaraldehyde. The routine procedures of preparation of tissues were followed for light and transmission electron microscopic study. In macrosco...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- The Biochemical journal
دوره 209 1 شماره
صفحات -
تاریخ انتشار 1983